PrimePCR™ SYBR® Green Assay: MAN2B1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050290

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAN2B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAN2B1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043028
Assay Design:   exonic
Chromosome Location:   19:12774568-12775629question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000447393 ENST00000547217 ENST00000458164 ENST00000325438 ENST00000544334 ENST00000306801 ENST00000338335 ENST00000456935 ENST00000221363 ENST00000486847 ENST00000371941 ENST00000396220 ENST00000358965 ENST00000393695 ENST00000542977 ENST00000537217 ENST00000367240 ENST00000447715 ENST00000295706 ENST00000247226 ENST00000394691 ENST00000471182 ENST00000484731 ENST00000355035 ENST00000254850 ENST00000574868 ENST00000380952 ENST00000447654 ENST00000340950 ENST00000284548 ENST00000570156 ENST

Gene Information

This gene encodes an enzyme that hydrolyzes terminal non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa 42 kDa (D) and 13/15 kDa (E). Next the 70 kDa peptide is further processed into three peptides (A B and C). The A B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2010]

Gene Symbol:   MAN2B1
Gene Name:   mannosidase, alpha, class 2B, member 1
Aliases:   LAMAN, MANB
RefSeq:   NC_000019.9 NG_008318.1 NG_015814.1 NT_011295.11
Ensembl:   ENSG00000104774
Entrez:   4125
Chromosome Mapping:   19cen-q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.470000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download