This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0050281
PrimePCR™ PreAmp for SYBR® Green Assay: SLC39A14, Human
PrimePCR™ Template for SYBR® Green Assay: SLC39A14, Human
Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein nucleic acid carbohydrate and lipid metabolism as well as in the control of gene transcription growth development and differentiation. SLC39A14 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson 2003 [PubMed 12659941]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.