PrimePCR™ SYBR® Green Assay: RP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050260

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042998
Assay Design:   exonic
Chromosome Location:   8:55540292-55540413question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000220676

Gene Information

This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains which bind microtubules and regulate microtubule polymerization. The encoded protein is a photoreceptor microtubule-associated protein and is required for correct stacking of outer segment disc. This protein and the RP1L1 protein another retinal-specific protein play essential and synergistic roles in affecting photosensitivity and outer segment morphogenesis of rod photoreceptors. Because of its response to in vivo retinal oxygen levels this protein was initially named ORP1 (oxygen-regulated protein-1). This protein was subsequently designated RP1 (retinitis pigmentosa 1) when it was found that mutations in this gene cause autosomal dominant retinitis pigmentosa. Mutations in this gene also cause autosomal recessive retinitis pigmentosa. Transcript variants resulted from an alternative promoter and alternative splicings have been found which overlap the current reference sequence and has several exons upstream and downstream of the current reference sequence. However the biological validity and full-length nature of some variants cannot be determined at this time.[provided by RefSeq Sep 2010]

Gene Symbol:   RP1
Gene Name:   retinitis pigmentosa 1 (autosomal dominant)
Aliases:   DCDC4A, FLJ50293, FLJ55454, FLJ79410, ORP1
RefSeq:   NC_000008.10 NG_009840.1 NT_008183.19
Ensembl:   ENSG00000104237
Entrez:   6101
UniGene:   Hs.732820
Chromosome Mapping:   8q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.280000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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