PrimePCR™ SYBR® Green Assay: ZFAND1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050259

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZFAND1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZFAND1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042997
Assay Design:   exonic
Chromosome Location:   8:82627222-82627343question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000523096 ENST00000220669 ENST00000522520 ENST00000521895 ENST00000521287 ENST00000520635 ENST00000517588 ENST00000519523 ENST00000520604 ENST00000523361 ENST00000517450 ENST00000521742 ENST00000518419 ENST00000520076

Gene Information

Description Not Available

Gene Symbol:   ZFAND1
Gene Name:   zinc finger, AN1-type domain 1
Aliases:   FLJ14007
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000104231
Entrez:   79752
Chromosome Mapping:   8q21.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download