PrimePCR™ SYBR® Green Assay: HMOX2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050235

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HMOX2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HMOX2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042973
Assay Design:   exonic
Chromosome Location:   16:4556925-4557729question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000486442 ENST00000449193 ENST00000575051 ENST00000570646 ENST00000458134 ENST00000219700 ENST00000575120 ENST00000572812 ENST00000574466 ENST00000576827 ENST00000570445 ENST00000575129 ENST00000398595 ENST00000406590 ENST00000414777 ENST00000493030

Gene Information

Heme oxygenase an essential enzyme in heme catabolism cleaves heme to form biliverdin which is subsequently converted to bilirubin by biliverdin reductase and carbon monoxide a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Alternative splice variants encoding the same protein have been identified at this locus. [provided by RefSeq Jul 2008]

Gene Symbol:   HMOX2
Gene Name:   heme oxygenase (decycling) 2
Aliases:   HO-2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103415
Entrez:   3163
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.230000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download