PrimePCR™ SYBR® Green Assay: DHRS12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050203

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042941
Assay Design:   exonic
Chromosome Location:   13:52373671-52373797question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000444610 ENST00000218981 ENST00000280056

Gene Information

Description Not Available

Gene Symbol:   DHRS12
Gene Name:   dehydrogenase/reductase (SDR family) member 12
Aliases:   FLJ13639, SDR40C1
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000102796
Entrez:   79758
UniGene:   Hs.266728
Chromosome Mapping:   13q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.690000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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