PrimePCR™ SYBR® Green Assay: KATNAL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050202

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042940
Assay Design:   exonic
Chromosome Location:   13:30857788-30881476question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000380615 ENST00000380617 ENST00000414289 ENST00000441394

Gene Information

Description Not Available

Gene Symbol:   KATNAL1
Gene Name:   katanin p60 subunit A-like 1
Aliases:   MGC2599
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000102781
Entrez:   84056
UniGene:   Hs.243596
Chromosome Mapping:   13q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.190000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download