PrimePCR™ SYBR® Green Assay: NALCN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050188

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NALCN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NALCN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042926
Assay Design:   exonic
Chromosome Location:   13:102029274-102029404question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000251127 ENST00000376196 ENST00000376200

Gene Information

NALCN forms a voltage-independent nonselective noninactivating cation channel permeable to Na+ K+ and Ca(2+). It is responsible for the neuronal background sodium leak conductance (Lu et al. 2007 [PubMed 17448995]).[supplied by OMIM Mar 2008]

Gene Symbol:   NALCN
Gene Name:   sodium leak channel, non-selective
Aliases:   CanIon, FLJ23913, FLJ44659, FLJ44764, MGC74524, VGCNL1, bA430M15.1
RefSeq:   NC_000013.10 NT_009952.14
Ensembl:   ENSG00000102452
Entrez:   259232
Chromosome Mapping:   13q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.910000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download