PrimePCR™ SYBR® Green Assay: PHEX, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0050176

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042914
Assay Design:   exonic
Chromosome Location:   X:22266011-22266130question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000379374 ENST00000537599 ENST00000535894 ENST00000418858

Gene Information

The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. [provided by RefSeq Jul 2008]

Gene Symbol:   PHEX
Gene Name:   phosphate regulating endopeptidase homolog, X-linked
Aliases:   HPDR, HPDR1, HYP, HYP1, LXHR, PEX, XLH
RefSeq:   NC_000023.10 NG_007563.1 NT_167197.1
Ensembl:   ENSG00000102174
Entrez:   5251
UniGene:   Hs.495834
Chromosome Mapping:   Xp22.2-p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.880000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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