PrimePCR™ SYBR® Green Assay: KCND1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050165

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KCND1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KCND1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042903
Assay Design:   exonic
Chromosome Location:   X:48822587-48822711question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000334025 ENST00000303415 ENST00000381583 ENST00000290607 ENST00000284674 ENST00000407004 ENST00000589998 ENST00000592763 ENST00000341083 ENST00000376477 ENST00000218176 ENST00000419374 ENST00000318443 ENST00000561213 ENST00000261842 ENST00000560508 ENST00000084798 ENST00000600735 ENST00000593940 ENST00000596377

Gene Information

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release heart rate insulin secretion neuronal excitability epithelial electrolyte transport smooth muscle contraction and cell volume. Four sequence-related potassium channel genes - shaker shaw shab and shal - have been identified in Drosophila and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel voltage-gated shal-related subfamily members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This gene is expressed at moderate levels in all tissues analyzed with lower levels in skeletal muscle. [provided by RefSeq Jul 2008]

Gene Symbol:   KCND1
Gene Name:   potassium voltage-gated channel, Shal-related subfamily, member 1
Aliases:   KV4.1
RefSeq:   NC_000023.10 NG_012515.1 NG_016263.1 NT_079573.4
Ensembl:   ENSG00000102057
Entrez:   3750
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download