PrimePCR™ SYBR® Green Assay: ELF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050162

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042900
Assay Design:   exonic
Chromosome Location:   X:129208595-129208693question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000335997 ENST00000308167 ENST00000434609

Gene Information

The protein encoded by this gene is a transcriptional activator that binds and activates the promoters of the CSF2 IL3 IL8 and PRF1 genes. The encoded protein is involved in natural killer cell development and function innate immunity and induction of cell cycle arrest in naive CD8+ cells. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq Jan 2010]

Gene Symbol:   ELF4
Gene Name:   E74-like factor 4 (ets domain transcription factor)
Aliases:   ELFR, MEF
RefSeq:   NC_000023.10 NT_011786.16 NG_016388.1
Ensembl:   ENSG00000102034
Entrez:   2000
UniGene:   Hs.271940
Chromosome Mapping:   Xq26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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