PrimePCR™ SYBR® Green Assay: H2BFM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050146

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042884
Assay Design:   exonic
Chromosome Location:   X:103296760-103296893question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000247226 ENST00000394691 ENST00000471182 ENST00000484731 ENST00000217926 ENST00000355016 ENST00000417637 ENST00000243297 ENST00000598499 ENST00000596101 ENST00000596976 ENST00000597637

Gene Information

Description Not Available

Gene Symbol:   H2BFM
Gene Name:   H2B histone family, member M
Aliases:   Not Available
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000101812
Entrez:   286436
UniGene:   Hs.376474
Chromosome Mapping:   Xq22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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