PrimePCR™ SYBR® Green Assay: CSTF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050145

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042883
Assay Design:   exonic
Chromosome Location:   X:100078321-100078416question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000372972 ENST00000413437 ENST00000415585

Gene Information

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq Jul 2008]

Gene Symbol:   CSTF2
Gene Name:   cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Aliases:   CstF-64
RefSeq:   NC_000023.10 NG_015848.1 NT_011651.17
Ensembl:   ENSG00000101811
Entrez:   1478
UniGene:   Hs.132370
Chromosome Mapping:   Xq22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.730000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download