PrimePCR™ SYBR® Green Assay: CEP192, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050140

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042878
Assay Design:   exonic
Chromosome Location:   18:13059127-13059276question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000589596 ENST00000506447 ENST00000511820 ENST00000430049 ENST00000325971

Gene Information

Description Not Available

Gene Symbol:   CEP192
Gene Name:   centrosomal protein 192kDa
Aliases:   Not Available
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000101639
Entrez:   55125
UniGene:   Hs.100914
Chromosome Mapping:   18p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.480000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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