PrimePCR™ SYBR® Green Assay: GTPBP5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050106

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042844
Assay Design:   exonic
Chromosome Location:   20:60770871-60772933question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000370823 ENST00000448254 ENST00000436421 ENST00000536470

Gene Information

Small G proteins such as GTPBP5 act as molecular switches that play crucial roles in the regulation of fundamental cellular processes such as protein synthesis nuclear transport membrane trafficking and signal transduction (Hirano et al. 2006 [PubMed 17054726]).[supplied by OMIM Mar 2008]

Gene Symbol:   GTPBP5
Gene Name:   GTP binding protein 5 (putative)
Aliases:   FLJ10741, MGC29512, ObgH1, dJ1005F21.2
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101181
Entrez:   26164
UniGene:   Hs.340636
Chromosome Mapping:   20q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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