PrimePCR™ SYBR® Green Assay: NINL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050100

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042838
Assay Design:   exonic
Chromosome Location:   20:25443044-25443174question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000278886 ENST00000336104 ENST00000422516

Gene Information

Description Not Available

Gene Symbol:   NINL
Gene Name:   ninein-like
Aliases:   FLJ11792, KIAA0980, NLP, dJ691N24.1
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000101004
Entrez:   22981
UniGene:   Hs.631508
Chromosome Mapping:   20p11.22-p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.360000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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