PrimePCR™ SYBR® Green Assay: TCL1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050078

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042816
Assay Design:   exonic
Chromosome Location:   14:96176770-96176859question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000331285 ENST00000570391 ENST00000554012 ENST00000556450 ENST00000557043 ENST00000402399

Gene Information

Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha (MIM 186880) or TCR-beta (MIM 186930) regulatory elements (summarized by Virgilio et al. 1998 [PubMed 9520462]). In normal T cells TCL1 is expressed in CD4-/CD8- cells but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (MIM 164730) (Laine et al. 2000 [PubMed 10983986]).[supplied by OMIM Jul 2010]

Gene Symbol:   TCL1A
Gene Name:   T-cell leukemia/lymphoma 1A
Aliases:   TCL1
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100721
Entrez:   8115
UniGene:   Hs.2484
Chromosome Mapping:   14q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download