PrimePCR™ SYBR® Green Assay: SRSF5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050076

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042814
Assay Design:   exonic
Chromosome Location:   14:70234876-70234989question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000553521 ENST00000394366 ENST00000553548 ENST00000553369 ENST00000557154 ENST00000553635 ENST00000555349 ENST00000554021 ENST00000451983

Gene Information

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Sep 2010]

Gene Symbol:   SRSF5
Gene Name:   serine/arginine-rich splicing factor 5
Aliases:   HRS, SFRS5, SRP40
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100650
Entrez:   6430
UniGene:   Hs.632326
Chromosome Mapping:   14q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.150000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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