This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0050072
PrimePCR™ PreAmp for SYBR® Green Assay: CHGA, Human
PrimePCR™ Template for SYBR® Green Assay: CHGA, Human
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin pancreastatin and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides including chromostatin beta-granin WE-14 and GE-25 are also derived from the full-length protein. However biological activities for these molecules have not been shown. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.