PrimePCR™ SYBR® Green Assay: RBM23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050053

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: RBM23, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RBM23, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042791
Assay Design:   exonic
Chromosome Location:   14:23388213-23388326question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000277540 ENST00000559228 ENST00000450403 ENST00000371092 ENST00000271002 ENST00000555209 ENST00000359890 ENST00000399922 ENST00000346528 ENST00000542016 ENST00000557403 ENST00000555722 ENST00000557227 ENST00000554256 ENST00000557549 ENST00000555676 ENST00000557571 ENST00000556862 ENST00000557464 ENST00000554618 ENST00000439151 ENST00000347982 ENST00000508029 ENST00000503056 ENST00000504457 ENST00000505395 ENST00000515735 ENST00000361032 ENST00000354179

Gene Information

This gene encodes a member of the U2AF-like family of RNA binding proteins. This protein interacts with some steroid nuclear receptors localizes to the promoter of a steroid- responsive gene and increases transcription of steroid-responsive transcriptional reporters in a hormone-dependent manner. It is also implicated in the steroid receptor-dependent regulation of alternative splicing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RBM23
Gene Name:   RNA binding motif protein 23
Aliases:   CAPERbeta, FLJ10482, MGC4458, RNPC4
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100461
Entrez:   55147
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.760000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download