PrimePCR™ SYBR® Green Assay: ACO2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050046

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ACO2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACO2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042784
Assay Design:   exonic
Chromosome Location:   22:41903852-41907890question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000406486 ENST00000401395 ENST00000358431 ENST00000216254 ENST00000396512 ENST00000330342 ENST00000563907 ENST00000234389 ENST00000348943 ENST00000310483 ENST00000413622

Gene Information

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15) also known as Lon protease after oxidative modification. [provided by RefSeq Jul 2008]

Gene Symbol:   ACO2
Gene Name:   aconitase 2, mitochondrial
Aliases:   ACONM, MGC20605, MGC33908
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100412
Entrez:   50
Chromosome Mapping:   22q11.2-q13.31,22q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.410000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download