PrimePCR™ SYBR® Green Assay: RAB36, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050018

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042756
Assay Design:   exonic
Chromosome Location:   22:23487731-23492259question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000358864 ENST00000440097 ENST00000273317 ENST00000399262 ENST00000399251 ENST00000338040 ENST00000242317 ENST00000263116 ENST00000341989 ENST00000555006 ENST00000261884 ENST00000333572 ENST00000423258 ENST00000420344

Gene Information

Description Not Available

Gene Symbol:   RAB36
Gene Name:   RAB36, member RAS oncogene family
Aliases:   Not Available
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100228
Entrez:   9609
UniGene:   Hs.369557
Chromosome Mapping:   22q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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