This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0050003
PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A1, Human
PrimePCR™ Template for SYBR® Green Assay: SLC25A1, Human
The mitochondrial tricarboxylate transporter (also called citrate transport protein or CTP) is responsible for the movement of citrate across the mitochondrial inner membrane (Kaplan et al. 1993 [PubMed 8514800]).[supplied by OMIM Jan 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.