PrimePCR™ SYBR® Green Assay: SLC35E4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050001

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042739
Assay Design:   exonic
Chromosome Location:   22:31032385-31032492question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000576273 ENST00000343605 ENST00000406566 ENST00000300385 ENST00000440501 ENST00000321666 ENST00000421924 ENST00000340305 ENST00000368761 ENST00000359831 ENST00000340026 ENST00000392556

Gene Information

Description Not Available

Gene Symbol:   SLC35E4
Gene Name:   solute carrier family 35, member E4
Aliases:   MGC129826
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100036
Entrez:   339665
UniGene:   Hs.660384
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.860000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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