PrimePCR™ SYBR® Green Assay: SEC14L3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049995

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042733
Assay Design:   exonic
Chromosome Location:   22:30858090-30860826question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000403066 ENST00000215812 ENST00000402286 ENST00000401751 ENST00000435069 ENST00000415957 ENST00000539629 ENST00000540910

Gene Information

The protein encoded by this gene is highly similar to the protein encoded by the Saccharomyces cerevisiae SEC14 gene. The SEC14 protein is a phophatidylinositol transfer protein that is essential for biogenesis of Golgi-derived transport vesicles and thus is required for the export of yeast secretory proteins from the Golgi complex. The specific function of this protein has not yet been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   SEC14L3
Gene Name:   SEC14-like 3 (S. cerevisiae)
Aliases:   MGC119366, MGC119367, MGC119368, TAP2
RefSeq:   NC_000022.10 NG_027515.1 NT_011520.12
Ensembl:   ENSG00000100012
Entrez:   266629
UniGene:   Hs.505601
Chromosome Mapping:   22q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.450000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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