This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049967
PrimePCR™ PreAmp for SYBR® Green Assay: SCD, Human
PrimePCR™ Template for SYBR® Green Assay: SCD, Human
This gene encodes an enzyme involved in fatty acid biosynthesis primarily the synthesis of oleic acid. Transcripts of approximately 3.9 and 5.2 kb differing only by alternative polyadenlyation signals have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq Feb 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.