PrimePCR™ SYBR® Green Assay: BRPF3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049955

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042693
Assay Design:   exonic
Chromosome Location:   6:36168212-36168345question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000357641 ENST00000339717 ENST00000534694 ENST00000446974 ENST00000454960 ENST00000534400 ENST00000543502 ENST00000443324

Gene Information

Description Not Available

Gene Symbol:   BRPF3
Gene Name:   bromodomain and PHD finger containing, 3
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000096070
Entrez:   27154
UniGene:   Hs.520096
Chromosome Mapping:   6p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.430000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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