PrimePCR™ SYBR® Green Assay: SEMA4G, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049947

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SEMA4G, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SEMA4G, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042685
Assay Design:   exonic
Chromosome Location:   10:102732914-102733307question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000519649 ENST00000518124 ENST00000370250 ENST00000517724 ENST00000210633

Gene Information

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities semaphorins are put into eight classes: invertebrates contain classes 1 and 2 viruses have class V and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:   SEMA4G
Gene Name:   sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G
Aliases:   FLJ20590, KIAA1619, MGC102867
RefSeq:   NC_000010.10 NG_011646.1 NG_012624.1 NT_030059.13
Ensembl:   ENSG00000095539
Entrez:   57715
Chromosome Mapping:   10q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.060000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download