PrimePCR™ SYBR® Green Assay: PTGS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049942

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042680
Assay Design:   exonic
Chromosome Location:   9:125154551-125154680question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000223423 ENST00000362012 ENST00000540753 ENST00000373698

Gene Information

Prostaglandin-endoperoxide synthase (PTGS) also known as cyclooxygenase is the key enzyme in prostaglandin biosynthesis and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2 which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1 which regulates angiogenesis in endothelial cells and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq Jul 2008]

Gene Symbol:   PTGS1
Gene Name:   prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase)
Aliases:   COX1, COX3, PCOX1, PGG/HS, PGHS-1, PGHS1, PHS1, PTGHS
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000095303
Entrez:   5742
UniGene:   Hs.201978
Chromosome Mapping:   9q32-q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.590000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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