PrimePCR™ SYBR® Green Assay: ARCN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049940

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042678
Assay Design:   exonic
Chromosome Location:   11:118473062-118473175question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000359415 ENST00000534182 ENST00000264028 ENST00000594860 ENST00000595576 ENST00000596715

Gene Information

This gene maps in a region which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins and may be involved in vesicle structure or trafficking. [provided by RefSeq Jul 2008]

Gene Symbol:   ARCN1
Gene Name:   archain 1
Aliases:   COPD
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000095139
Entrez:   372
UniGene:   Hs.33642
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.590000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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