PrimePCR™ SYBR® Green Assay: CECR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049932

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042670
Assay Design:   exonic
Chromosome Location:   22:17663553-17663648question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000399839 ENST00000330232 ENST00000262607 ENST00000399837 ENST00000449907

Gene Information

This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein may act as a growth factor and have adenosine deaminase activity. It may be responsible for some of the phenotypic features associated with cat eye syndrome. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CECR1
Gene Name:   cat eye syndrome chromosome region, candidate 1
Aliases:   ADA2, ADGF, IDGFL
RefSeq:   NC_000022.10 NT_011519.10
Ensembl:   ENSG00000093072
Entrez:   51816
UniGene:   Hs.170310
Chromosome Mapping:   22q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.200000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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