PrimePCR™ SYBR® Green Assay: SEMA6A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049921

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042659
Assay Design:   exonic
Chromosome Location:   5:115782290-115782430question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000343348 ENST00000257414 ENST00000513137 ENST00000515129 ENST00000503865 ENST00000510263 ENST00000282394

Gene Information

The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al. 2001 [PubMed 11242070]).[supplied by OMIM Feb 2011]

Gene Symbol:   SEMA6A
Gene Name:   sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Aliases:   HT018, KIAA1368, SEMA, SEMA6A1, SEMAQ, VIA
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000092421
Entrez:   57556
UniGene:   Hs.156967
Chromosome Mapping:   5q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.600000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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