PrimePCR™ SYBR® Green Assay: RPGRIP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049916

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RPGRIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RPGRIP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042654
Assay Design:   exonic
Chromosome Location:   14:21798436-21802780question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000556336 ENST00000557771 ENST00000400017 ENST00000382933 ENST00000555587 ENST00000557606 ENST00000206660 ENST00000307974

Gene Information

This gene encodes a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. Mutations in this gene lead to autosomal recessive congenital blindness. [provided by RefSeq Oct 2008]

Gene Symbol:   RPGRIP1
Gene Name:   retinitis pigmentosa GTPase regulator interacting protein 1
Aliases:   CORD13, DKFZp686P0897, LCA6, RGI1, RGRIP, RPGRIP, RPGRIP1d
RefSeq:   NC_000014.8 NG_008933.1 NG_009932.1 NT_026437.12
Ensembl:   ENSG00000092200
Entrez:   57096
Chromosome Mapping:   14q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download