PrimePCR™ SYBR® Green Assay: SCFD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049913

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042651
Assay Design:   exonic
Chromosome Location:   14:31119831-31122787question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000541123 ENST00000421551 ENST00000469043 ENST00000396629 ENST00000544052 ENST00000458591

Gene Information

Description Not Available

Gene Symbol:   SCFD1
Gene Name:   sec1 family domain containing 1
Aliases:   C14orf163, KIAA0917, RA410, SLY1, STXBP1L2
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000092108
Entrez:   23256
UniGene:   Hs.369168
Chromosome Mapping:   14q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.640000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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