This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0049903
PrimePCR™ PreAmp for SYBR® Green Assay: ESR1, Human
PrimePCR™ Template for SYBR® Green Assay: ESR1, Human
This gene encodes an estrogen receptor a ligand-activated transcription factor composed of several domains important for hormone binding DNA binding and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer endometrial cancer and osteoporosis. Alternative splicing results in several transcript variants which differ in their 5' UTRs and use different promoters. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.