PrimePCR™ SYBR® Green Assay: DNAJB11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049873

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042611
Assay Design:   exonic
Chromosome Location:   3:186301655-186302235question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000439351 ENST00000418776 ENST00000265028

Gene Information

DNAJB11 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a C-terminal cysteine-rich region (Ohtsuka and Hata 2000 [PubMed 11147971]).[supplied by OMIM Mar 2008]

Gene Symbol:   DNAJB11
Gene Name:   DnaJ (Hsp40) homolog, subfamily B, member 11
Aliases:   ABBP-2, ABBP2, DJ9, EDJ, ERdj3, ERj3, HEDJ, PRO1080, UNQ537, hDj9
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000090520
Entrez:   51726
UniGene:   Hs.317192
Chromosome Mapping:   3q27.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.490000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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