PrimePCR™ SYBR® Green Assay: TRMT6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049858

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TRMT6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TRMT6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042596
Assay Design:   exonic
Chromosome Location:   20:5923200-5923346question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000203001 ENST00000453074

Gene Information

The protein encoded by this gene is similar to an uncharacterized protein from C. elegans. Although the function of the encoded protein is unknown it shares weak sequence similarity to a region of S. cerevisiae translation initiation factor subunit Gcd10p. [provided by RefSeq Jul 2008]

Gene Symbol:   TRMT6
Gene Name:   tRNA methyltransferase 6 homolog (S. cerevisiae)
Aliases:   GCD10, Gcd10p, MGC5029
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000089195
Entrez:   51605
Chromosome Mapping:   20p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.380000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download