PrimePCR™ SYBR® Green Assay: KIF9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049841

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042579
Assay Design:   exonic
Chromosome Location:   3:47316832-47316945question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000265529 ENST00000444589 ENST00000452770 ENST00000456548 ENST00000425853 ENST00000432493 ENST00000425452 ENST00000335044 ENST00000352910

Gene Information

Description Not Available

Gene Symbol:   KIF9
Gene Name:   kinesin family member 9
Aliases:   MGC104186
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000088727
Entrez:   64147
UniGene:   Hs.373947
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.740000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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