PrimePCR™ SYBR® Green Assay: CNOT3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049835

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CNOT3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CNOT3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042573
Assay Design:   exonic
Chromosome Location:   19:54651892-54652021question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000430939 ENST00000262325 ENST00000319441 ENST00000543666 ENST00000221232 ENST00000406403 ENST00000440571 ENST00000358389 ENST00000373995 ENST00000395489 ENST00000373256 ENST00000315869 ENST00000563500 ENST00000299138 ENST00000360458 ENST00000441076 ENST00000433176 ENST00000422154 ENST00000451301 ENST00000423833 ENST00000409509 ENST00000409037 ENST00000393992 ENST00000424285 ENST00000341546 ENST00000355530 ENST00000342232 ENST00000601543 ENST00000576593 ENST00000598781 ENST00000601960 ENST

Gene Information

Description Not Available

Gene Symbol:   CNOT3
Gene Name:   CCR4-NOT transcription complex, subunit 3
Aliases:   KIAA0691, LENG2, NOT3, NOT3H
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000088038
Entrez:   4849
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.070000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download