PrimePCR™ SYBR® Green Assay: PIGS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049819

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042557
Assay Design:   exonic
Chromosome Location:   17:26881025-26881139question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000395346 ENST00000308360 ENST00000543734

Gene Information

This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   PIGS
Gene Name:   phosphatidylinositol glycan anchor biosynthesis, class S
Aliases:   DKFZp686K20216, FLJ45226
RefSeq:   NC_000017.10 NG_012302.1 NT_010799.15
Ensembl:   ENSG00000087111
Entrez:   94005
UniGene:   Hs.462550
Chromosome Mapping:   17p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.300000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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