PrimePCR™ SYBR® Green Assay: FAT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049808

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042546
Assay Design:   exonic
Chromosome Location:   5:150900976-150901108question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000520200 ENST00000261800

Gene Information

This gene is the second identified human homolog of the Drosophila fat gene which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats the gene product has two epidermal growth factor (EGF)-like repeats and one laminin G domain. This protein most likely functions as a cell adhesion molecule controlling cell proliferation and playing an important role in cerebellum development. [provided by RefSeq Jul 2008]

Gene Symbol:   FAT2
Gene Name:   FAT tumor suppressor homolog 2 (Drosophila)
Aliases:   CDHF8, CDHR9, HFAT2, MEGF1
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000086570
Entrez:   2196
UniGene:   Hs.591255
Chromosome Mapping:   5q33.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.060000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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