PrimePCR™ SYBR® Green Assay: FYB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049738

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FYB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FYB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042476
Assay Design:   exonic
Chromosome Location:   5:39202902-39202999question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000576273 ENST00000592419 ENST00000457710 ENST00000440501 ENST00000321666 ENST00000369774 ENST00000355946 ENST00000421573 ENST00000321871 ENST00000393130 ENST00000515826 ENST00000367262 ENST00000369452 ENST00000265277 ENST00000329476 ENST00000506608 ENST00000351578 ENST00000515010 ENST00000505428 ENST00000512982 ENST00000510188 ENST00000512138 ENST00000506557 ENST00000540520 ENST00000519546

Gene Information

The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   FYB
Gene Name:   FYN binding protein
Aliases:   ADAP, PRO0823, SLAP-130
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000082074
Entrez:   2533
Chromosome Mapping:   5p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download