PrimePCR™ SYBR® Green Assay: ZNF506, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049733

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF506, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF506, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042471
Assay Design:   exonic
Chromosome Location:   19:19916931-19932413question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000587461 ENST00000590766 ENST00000540806 ENST00000450683 ENST00000587452 ENST00000545006 ENST00000443905 ENST00000589717 ENST00000591366 ENST00000592160 ENST00000343769 ENST00000418063 ENST00000597083 ENST00000335117 ENST00000427401 ENST00000596797 ENST00000601440 ENST00000291750 ENST00000360204 ENST00000594534 ENST00000344519 ENST00000425625 ENST00000597314 ENST00000601924 ENST00000300540 ENST00000595854 ENST00000601284 ENST00000328178 ENST00000596476 ENST00000345030 ENST00000261560 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF506
Gene Name:   zinc finger protein 506
Aliases:   DKFZp761G1812, MGC27136
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000081665
Entrez:   440515
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 39.190000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download