PrimePCR™ SYBR® Green Assay: SP140, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049697

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042435
Assay Design:   exonic
Chromosome Location:   2:231102938-231103041question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000392045 ENST00000417495 ENST00000343805 ENST00000373645 ENST00000486687 ENST00000420434 ENST00000350136

Gene Information

Description Not Available

Gene Symbol:   SP140
Gene Name:   SP140 nuclear body protein
Aliases:   LYSP100, LYSP100-A, LYSP100-B, MGC126440
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000079263
Entrez:   11262
UniGene:   Hs.632549
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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