PrimePCR™ SYBR® Green Assay: PKD2L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049688

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042426
Assay Design:   exonic
Chromosome Location:   5:137230095-137230244question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000350250 ENST00000511176 ENST00000290431 ENST00000508883 ENST00000502810 ENST00000508638 ENST00000503015

Gene Information

Description Not Available

Gene Symbol:   PKD2L2
Gene Name:   polycystic kidney disease 2-like 2
Aliases:   TRPP5
RefSeq:   NC_000005.9 NG_008894.1 NT_034772.6
Ensembl:   ENSG00000078795
Entrez:   27039
UniGene:   Hs.716884
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.650000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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