This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0049675
PrimePCR™ PreAmp for SYBR® Green Assay: N4BP2, Human
PrimePCR™ Template for SYBR® Green Assay: N4BP2, Human
This gene encodes a protein containing a polynucleotide kinase domain (PNK) near the N-terminal region and a Small MutS Related (Smr) domain near the C-terminal region. The encoded protein can bind to both B-cell leukemia/lymphoma 3 (BCL-3) and neural precursor cell expressed developmentally downregulated 4 (Nedd4) proteins. This protein binds and hydrolyzes ATP may function as a 5'-polynucleotide kinase and has the capacity to be a ubiquitylation substrate. This protein may play a role in transcription-coupled DNA repair or genetic recombination. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.