PrimePCR™ SYBR® Green Assay: LAMP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049673

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042411
Assay Design:   exonic
Chromosome Location:   3:182871928-182872070question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000470251 ENST00000476015 ENST00000466939 ENST00000265598

Gene Information

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al. 1998 [PubMed 9768752]).[supplied by OMIM Dec 2010]

Gene Symbol:   LAMP3
Gene Name:   lysosomal-associated membrane protein 3
Aliases:   CD208, DC LAMP, DC-LAMP, DCLAMP, LAMP, LAMP-3, TSC403
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000078081
Entrez:   27074
UniGene:   Hs.518448
Chromosome Mapping:   3q26.3-q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.340000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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