PrimePCR™ SYBR® Green Assay: GTF2I, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049668

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GTF2I, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GTF2I, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042406
Assay Design:   exonic
Chromosome Location:   7:74105325-74105433question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000324896 ENST00000353920 ENST00000346152 ENST00000416070 ENST00000443166 ENST00000432143 ENST00000405086 ENST00000361071 ENST00000417115 ENST00000453619 ENST00000356115 ENST00000312575 ENST00000430511 ENST00000573035

Gene Information

This gene encodes a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 7 13 and 21. [provided by RefSeq Jul 2009]

Gene Symbol:   GTF2I
Gene Name:   general transcription factor IIi
Aliases:   BAP135, BTKAP1, DIWS, FLJ38776, FLJ56355, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6
RefSeq:   NC_000007.13 NT_007933.15 NG_008179.1
Ensembl:   ENSG00000077809
Entrez:   2969
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.420000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download