PrimePCR™ SYBR® Green Assay: ANKRD13A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049648

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042386
Assay Design:   exonic
Chromosome Location:   12:110467433-110471612question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000217971 ENST00000261739 ENST00000547639 ENST00000553251 ENST00000549826 ENST00000551491

Gene Information

Description Not Available

Gene Symbol:   ANKRD13A
Gene Name:   ankyrin repeat domain 13A
Aliases:   ANKRD13, NY-REN-25
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000076513
Entrez:   88455
UniGene:   Hs.528703
Chromosome Mapping:   12q24.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.440000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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