PrimePCR™ SYBR® Green Assay: RGS11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049646

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RGS11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGS11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042384
Assay Design:   exonic
Chromosome Location:   16:323550-324051question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000431721 ENST00000449610 ENST00000446860 ENST00000417896 ENST00000443964 ENST00000434342 ENST00000563869 ENST00000248121 ENST00000562045 ENST00000320442 ENST00000379218 ENST00000397462 ENST00000397770 ENST00000316163 ENST00000359740 ENST00000397768 ENST00000397852 ENST00000397857 ENST00000397854 ENST00000355153 ENST00000397850 ENST00000302347 ENST00000258415 ENST00000366697 ENST00000366698 ENST00000295033 ENST00000456946 ENST00000479800 ENST00000355586 ENST00000442677 ENST00000398786 ENST

Gene Information

The protein encoded by this gene belongs to the RGS (regulator of G protein signaling) family. Members of the RGS family act as GTPase-activating proteins on the alpha subunits of heterotrimeric signal-transducing G proteins. This protein inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   RGS11
Gene Name:   regulator of G-protein signaling 11
Aliases:   RS11
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000076344
Entrez:   8786
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.510000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download